7o2e

X-ray diffraction
2.5Å resolution

Crystal structure of the human METTL3-METTL14 complex bound to Compound 21 (ADO_AD_089)

Released:

Function and Biology Details

Reaction catalysed:
S-adenosyl-L-methionine + adenine in mRNA = S-adenosyl-L-homocysteine + N(6)-methyladenine in mRNA
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
N6-adenosine-methyltransferase catalytic subunit Chain: A
Molecule details ›
Chain: A
Length: 246 amino acids
Theoretical weight: 28.14 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q86U44 (Residues: 354-580; Coverage: 39%)
Gene names: METTL3, MTA70
Sequence domains: MT-A70
N6-adenosine-methyltransferase non-catalytic subunit Chain: B
Molecule details ›
Chain: B
Length: 290 amino acids
Theoretical weight: 33.62 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9HCE5 (Residues: 106-395; Coverage: 64%)
Gene names: KIAA1627, METTL14
Sequence domains: MT-A70

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: P3221
Unit cell:
a: 63.93Å b: 63.93Å c: 224.6Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.209 0.206 0.273
Expression system: Spodoptera frugiperda