7o1o

X-ray diffraction
1.25Å resolution

Complex-B bound [FeFe]-hydrogenase maturase HydE fromT. Maritima (Auxiliary cluster deleted variant)

Released:
Source organism: Thermotoga maritima MSB8
Entry authors: Rohac R, Martin L, Liu L, Basu D, Tao L, Britt RD, Rauchfuss T, Nicolet Y

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
[FeFe] hydrogenase maturase subunit HydE Chain: A
Molecule details ›
Chain: A
Length: 357 amino acids
Theoretical weight: 40.88 KDa
Source organism: Thermotoga maritima MSB8
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q9X0Z6 (Residues: 2-345; Coverage: 99%)
Gene names: THEMA_07990, TM_1269, Tmari_1274
Sequence domains:

Ligands and Environments

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P21212
Unit cell:
a: 63.42Å b: 81.76Å c: 70.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.13 0.128 0.152
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'