› 7nuc

X-ray diffraction
2.77Å resolution

Crystal Structure of Neisseria gonorrhoeae LeuRS L550G mutant in Complex with ATP and L-leucinol

Released:
DOI: 10.2210/pdb7nuc/pdb
PDB 7nuc coloured by chain and viewed from the front.
PDB 7nuc coloured by chain and viewed from the side.
PDB 7nuc coloured by chain and viewed from the top.
Source organism: Neisseria gonorrhoeae
Primary publication:
Partitioning of the initial catalytic steps of leucyl-tRNA synthetase is driven by an active site peptide-plane flip.
Pang L, Zanki V, Strelkov SV, Van Aerschot A, Gruic-Sovulj I, Weeks SD
Commun Biol 5 883 (2022)
PMID: 36038645

Function and Biology Details

Reaction catalysed: 
ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 877 amino acids
Theoretical weight: 98.13 KDa
Source organism: Neisseria gonorrhoeae
Expression system: Escherichia coli
UniProt:
  • Canonical: B4RNT1 (Residues: 1-876; Coverage: 100%)
Gene names: NGK_0009, leuS
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand EDO 2 x EDO
Ligand ATP 1 x ATP
Ligand MG 2 x MG
Ligand DCL 1 x DCL
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 48.912Å b: 81.588Å c: 216.971Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.185 0.264
Expression system: Escherichia coli

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