7nuc

X-ray diffraction
2.77Å resolution

Crystal Structure of Neisseria gonorrhoeae LeuRS L550G mutant in Complex with ATP and L-leucinol

Released:

Function and Biology Details

Reaction catalysed:
ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Leucine--tRNA ligase Chain: A
Molecule details ›
Chain: A
Length: 877 amino acids
Theoretical weight: 98.13 KDa
Source organism: Neisseria gonorrhoeae
Expression system: Escherichia coli
UniProt:
  • Canonical: B4RNT1 (Residues: 1-876; Coverage: 100%)
Gene names: NGK_0009, leuS
Sequence domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 1
Spacegroup: P212121
Unit cell:
a: 48.912Å b: 81.588Å c: 216.971Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.185 0.264
Expression system: Escherichia coli