› 7npb

X-ray diffraction
1.37Å resolution

Crystal structure of 14-3-3 sigma in complex with 20mer Amot-p130 peptide and fragment 09

Released:
DOI: 10.2210/pdb7npb/pdb
PDB entry 7npb coloured by chain, front view.
PDB entry 7npb coloured by chain, side view.
PDB entry 7npb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Fragment-based exploration of the 14-3-3/Amot-p130 interface.
Centorrino F, Andlovic B, Cossar P, Brunsveld L, Ottmann C
Curr Res Struct Biol 4 21-28 (2022)
PMID: 35036934

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152160 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein sigma Chain: A
Molecule details ›
Chain: A
Length: 253 amino acids
Theoretical weight: 28.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P31947 (Residues: 1-248; Coverage: 100%)
Gene names: HME1, SFN
Sequence domains: 14-3-3 protein
Angiomotin Chain: P
Molecule details ›
Chain: P
Length: 20 amino acids
Theoretical weight: 2.24 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q4VCS5 (Residues: 169-188; Coverage: 2%)
Gene names: AMOT, KIAA1071

Ligands and Environments

3 bound ligands:
Ligand K7N 1 x K7N
Ligand CA 2 x CA
Ligand CL 1 x CL
2 modified residues:
Ligand CSO 1 x CSO
Ligand SEP 1 x SEP

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2221
Unit cell:
a: 82.177Å b: 111.826Å c: 62.766Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.156 0.154 0.179
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

1 review citation

Contemporary biophysical approaches for studying 14-3-3 protein-protein interactions.
Thurairajah et al. (2022)
1 other article

1 mention without citation

Fragment-based exploration of the 14-3-3/Amot-p130 interface.
Centorrino et al. (2022)