› 7nna

X-ray diffraction
1.9Å resolution

Toxin Import Through the Antibiotic Efflux Channel TolC

Released:
DOI: 10.2210/pdb7nna/pdb
PDB entry 7nna coloured by chain, front view.
PDB entry 7nna coloured by chain, side view.
PDB entry 7nna coloured by chain, top view.
Source organism: Klebsiella pneumoniae
Primary publication:
Toxin import through the antibiotic efflux channel TolC.
Housden NG, Webby MN, Lowe ED, El-Baba TJ, Kaminska R, Redfield C, Robinson CV, Kleanthous C
Nat Commun 12 4625 (2021)
PMID: 34330923

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178424 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Klebicin C activity Chain: A
Molecule details ›
Chain: A
Length: 212 amino acids
Theoretical weight: 23.8 KDa
Source organism: Klebsiella pneumoniae
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q5V9K0 (Residues: 52-254; Coverage: 33%)
Gene name: kca

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P3221
Unit cell:
a: 47.914Å b: 47.914Å c: 200.488Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.189 0.188 0.202
Expression system: Escherichia coli BL21

Quick links

Citations

3 review citations

Mass spectrometry of intact membrane proteins: shifting towards a more native-like context.
Oluwole et al. (2023)
2 more

1 mention without citation

Toxin import through the antibiotic efflux channel TolC.
Housden et al. (2021)