7nme

X-ray diffraction
2.2Å resolution

Human MHC Class I, A24 Allele presenting QLPRLFPLL, Complex with 4C6 TCR

Released:
Source organism: Homo sapiens
Entry authors: Rizkallah PJ, Sewell AK, Cole DK, Wall A

Function and Biology Details

Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 276 amino acids
Theoretical weight: 31.78 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A411J078 (Residues: 25-300; Coverage: 87%)
Gene name: HLA-A
Sequence domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
GLN-LEU-PRO-ARG-LEU-PHE-PRO-LEU-LEU Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 1.1 KDa
Source organism: Homo sapiens
Expression system: Not provided
4C6 Human T-cell Receptor, alpha Chain Chain: D
Molecule details ›
Chain: D
Length: 192 amino acids
Theoretical weight: 21.41 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
4C6 Human T-cell Receptor, beta Chain Chain: E
Molecule details ›
Chain: E
Length: 240 amino acids
Theoretical weight: 27.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 55.163Å b: 71.347Å c: 220.185Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.215 0.274
Expression systems:
  • Escherichia coli
  • Not provided