7nl1

X-ray diffraction
2.33Å resolution

Crystal structure of cystathionine gamma-lyase from Toxoplasma gondii

Released:
Source organism: Toxoplasma gondii ME49
Entry authors: Fernandez-Rodriguez C, Conter C, Recio I, Astegno A, Martinez-Cruz LA

Function and Biology Details

Reaction catalysed:
(1a) L-cystathionine = L-cysteine + 2-aminobut-2-enoate
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cystathionine gamma-lyase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 417 amino acids
Theoretical weight: 45.98 KDa
Source organism: Toxoplasma gondii ME49
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A125YN40 (Residues: 1-417; Coverage: 100%)
Gene name: TGME49_312930
Sequence domains: Cys/Met metabolism PLP-dependent enzyme

Ligands and Environments


Cofactor: Ligand PLP 8 x PLP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALBA BEAMLINE XALOC
Spacegroup: C2221
Unit cell:
a: 126.53Å b: 138.991Å c: 472.997Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.174 0.21
Expression system: Escherichia coli