› 7niu

Electron Microscopy
4.2Å resolution

Nanodisc reconstituted human ABCB4 in complex with 4B1-Fab and QA2-Fab (apo-inward-open conformation)

Released:
DOI: 10.2210/pdb7niu/pdb
PDB entry 7niu coloured by chain, front view.
PDB entry 7niu coloured by chain, side view.
PDB entry 7niu coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structures of ABCB4 provide insight into phosphatidylcholine translocation.
Nosol K, Bang-Sørensen R, Irobalieva RN, Erramilli SK, Stieger B, Kossiakoff AA, Locher KP
Proc Natl Acad Sci U S A 118 (2021)
PMID: 34385322
Related structures: EMD-12365

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + phospholipid(Side 1) = ADP + phosphate + phospholipid(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-210798 (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Phosphatidylcholine translocator ABCB4 Chain: A
Molecule details ›
Chain: A
Length: 1279 amino acids
Theoretical weight: 140.84 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
  • Canonical: P21439 (Residues: 1-1286; Coverage: 100%)
  • Best match: P21439-2 (Residues: 1-1279)
Gene names: ABCB4, MDR3, PGY3
Sequence domains:
QA2 Fab-fragment light chain Chain: D
Molecule details ›
Chain: D
Length: 215 amino acids
Theoretical weight: 23.26 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
QA2 Fab-fragment heavy chain Chain: E
Molecule details ›
Chain: E
Length: 234 amino acids
Theoretical weight: 24.82 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
4B1 Fab-fragment light chain Chain: B
Molecule details ›
Chain: B
Length: 215 amino acids
Theoretical weight: 23.35 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
4B1 Fab-fragment heavy chain Chain: C
Molecule details ›
Chain: C
Length: 241 amino acids
Theoretical weight: 25.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli

Ligands and Environments

1 bound ligand:
Ligand CLR 2 x CLR
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4.2Å
Relevant EMDB volumes: EMD-12365
Expression systems:
  • Homo sapiens
  • Escherichia coli

Quick links

Citations

4 review citations

Membranes under the Magnetic Lens: A Dive into the Diverse World of Membrane Protein Structures Using Cryo-EM.
Piper et al. (2022)
3 more

2 mentions without citation

Structural and functional insights of the human peroxisomal ABC transporter ALDP.
Jia et al. (2022)
1 more