› 7ney

X-ray diffraction
1.74Å resolution

Structure of T. atroviride Fdc wild-type (TaFdc) in complex with prFMN

Released:
DOI: 10.2210/pdb7ney/pdb
PDB entry 7ney coloured by chain, front view.
PDB entry 7ney coloured by chain, side view.
PDB entry 7ney coloured by chain, top view.
Source organism: Trichoderma atroviride IMI 206040
Primary publication:
Directed evolution of prenylated FMN-dependent Fdc supports efficient in vivo isobutene production.
Saaret A, Villiers B, Stricher F, Anissimova M, Cadillon M, Spiess R, Hay S, Leys D
Nat Commun 12 5300 (2021)
PMID: 34489427

Function and Biology Details

Reaction catalysed: 
4-coumarate = 4-vinylphenol + CO(2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-124689 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ferulic acid decarboxylase 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 519 amino acids
Theoretical weight: 56.95 KDa
Source organism: Trichoderma atroviride IMI 206040
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: G9NLP8 (Residues: 1-512; Coverage: 100%)
Gene names: FDC1, TRIATDRAFT_53567
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand 4LU 2 x 4LU
Ligand K 4 x K
Ligand MN 2 x MN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I24
Spacegroup: P3221
Unit cell:
a: 74.212Å b: 74.212Å c: 346.561Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.182 0.215
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 citations in other articles

Toolbox for the structure-guided evolution of ferulic acid decarboxylase (FDC).
Duță et al. (2022)

1 mention without citation

Directed evolution of prenylated FMN-dependent Fdc supports efficient in vivo isobutene production.
Saaret et al. (2021)