7ne6

X-ray diffraction
2.3Å resolution

Function and Biology Details

Reaction catalysed:
DNA 5-methylcytosine + 2-oxoglutarate + O(2) = DNA 5-hydroxymethylcytosine + succinate + CO(2) 
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Methylcytosine dioxygenase TET2 Chain: A
Molecule details ›
Chain: A
Length: 463 amino acids
Theoretical weight: 51.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6N021 (Residues: 1129-1480; Coverage: 18%)
  • Best match: Q6N021-3 (Residues: 1129-1136)
Gene names: KIAA1546, Nbla00191, TET2
Sequence domains: Oxygenase domain of the 2OGFeDO superfamily
DNA (5'-D(*AP*CP*AP*GP*GP*(5CM)P*GP*CP*CP*TP*G)-3') Chains: B, C
Molecule details ›
Chains: B, C
Length: 12 nucleotides
Theoretical weight: 3.68 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: C2221
Unit cell:
a: 47.858Å b: 87.943Å c: 261.988Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.227
Expression systems:
  • Escherichia coli
  • Not provided