› 7n7v

X-ray diffraction
1.99Å resolution

Crystal structure of TtnM, a Fe(II)-alpha-ketoglutarate-dependent hydroxylase from the tautomycetin biosynthesis pathway in Streptomyces griseochromogenes at 2 A.

Released:
DOI: 10.2210/pdb7n7v/pdb
PDB entry 7n7v coloured by chain, front view.
PDB entry 7n7v coloured by chain, side view.
PDB entry 7n7v coloured by chain, top view.
Source organism: Streptomyces griseochromogenes
Entry authors: Han L, Xu W, Ma M, Miller MD, Shen B, Phillips Jr GN, Enzyme Discovery for Natural Product Biosynthesis (NatPro)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-111665 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
TauD/TfdA-like domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 375 amino acids
Theoretical weight: 41.56 KDa
Source organism: Streptomyces griseochromogenes
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: C6ZCS6 (Residues: 1-339; Coverage: 100%)
Gene name: ttnM
Sequence domains: Taurine catabolism dioxygenase TauD, TfdA family

Ligands and Environments

2 bound ligands:
Ligand FE2 2 x FE2
Ligand CL 3 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID, APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 50.57Å b: 84.516Å c: 168.92Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.187 0.223
Expression system: Escherichia coli BL21(DE3)

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