› 7mww

X-ray diffraction
2.71Å resolution

Structure of hepatitis C virus envelope full-length glycoprotein 2 (eE2) from J6 genotype

Released:
DOI: 10.2210/pdb7mww/pdb
PDB entry 7mww coloured by chain, front view.
PDB entry 7mww coloured by chain, side view.
PDB entry 7mww coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural insights into hepatitis C virus receptor binding and entry.
Kumar A, Hossain RA, Yost SA, Bu W, Wang Y, Dearborn AD, Grakoui A, Cohen JI, Marcotrigiano J
Nature 598 521-525 (2021)
PMID: 34526719

Function and Biology Details

Reactions catalysed: 
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-207423 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Core protein precursor eE2 Chain: E
Molecule details ›
Chain: E
Length: 272 amino acids
Theoretical weight: 30.52 KDa
UniProt:
  • Canonical: A0A2I6PIY9 (Residues: 384-655; Coverage: 9%)
Sequence domains: Hepatitis C virus non-structural protein E2/NS1
2A12 Fab Heavy chain Chain: H
Molecule details ›
Chain: H
Length: 221 amino acids
Theoretical weight: 23.46 KDa
Source organism: Mus musculus
Expression system: Mus musculus
2A12 Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 240 amino acids
Theoretical weight: 26.55 KDa
Source organism: Mus musculus
Expression system: Mus musculus

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 2 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2221
Unit cell:
a: 95.84Å b: 155.561Å c: 129.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.218 0.27
Expression system: Mus musculus

Quick links

Citations

9 review citations

Production and immunogenicity of different prophylactic vaccines for hepatitis C virus (Review).
Zhao et al. (2022)
8 more

1 mention without citation

Regions of hepatitis C virus E2 required for membrane association.
Kumar et al. (2023)