7mww

X-ray diffraction
2.71Å resolution

Structure of hepatitis C virus envelope full-length glycoprotein 2 (eE2) from J6 genotype

Released:

Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
NTP + H(2)O = NDP + phosphate
Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (5 distinct):
Core protein precursor eE2 Chain: E
Molecule details ›
Chain: E
Length: 272 amino acids
Theoretical weight: 30.52 KDa
Source organism: Hepacivirus C
Expression system: Homo sapiens
UniProt:
  • Canonical: A0A2I6PIY1 (Residues: 384-655; Coverage: 9%)
2A12 Fab Heavy chain Chain: H
Molecule details ›
Chain: H
Length: 221 amino acids
Theoretical weight: 23.46 KDa
Source organism: Mus musculus
Expression system: Mus musculus
2A12 Fab light chain Chain: L
Molecule details ›
Chain: L
Length: 240 amino acids
Theoretical weight: 26.55 KDa
Source organism: Mus musculus
Expression system: Mus musculus

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA
Carbohydrate polymer : NEW Components: NAG
1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: C2221
Unit cell:
a: 95.84Å b: 155.561Å c: 129.42Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.218 0.27
Expression systems:
  • Homo sapiens
  • Mus musculus