7mwn

X-ray diffraction
1.9Å resolution

An engineered PYL2-based WIN 55,212-2 synthetic cannabinoid sensor with a stabilized HAB1 variant

Released:

Function and Biology Details

Reaction catalysed:
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Abscisic acid receptor PYL2 Chain: A
Molecule details ›
Chain: A
Length: 190 amino acids
Theoretical weight: 21.25 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O80992 (Residues: 1-190; Coverage: 100%)
Gene names: At2g26040, PYL2, RCAR14, T19L18.15
Sequence domains: Polyketide cyclase / dehydrase and lipid transport
Protein phosphatase 2C 16 Chain: B
Molecule details ›
Chain: B
Length: 333 amino acids
Theoretical weight: 36.72 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9CAJ0 (Residues: 179-511; Coverage: 68%)
Gene names: At1g72770, F28P22.4, HAB1, P2C-HA
Sequence domains: Protein phosphatase 2C

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P212121
Unit cell:
a: 61.326Å b: 69.226Å c: 142.817Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.185 0.211
Expression system: Escherichia coli BL21(DE3)