› 7mtb

Electron Microscopy
4Å resolution

Rhodopsin kinase (GRK1)-S5E/S488E/T489E in complex with rhodopsin and Fab6

Released:
DOI: 10.2210/pdb7mtb/pdb
PDB entry 7mtb coloured by chain, ensemble of 6 models, front view.
PDB entry 7mtb coloured by chain, ensemble of 6 models, side view.
PDB entry 7mtb coloured by chain, ensemble of 6 models, top view.
Source organisms:
Primary publication:
Structures of rhodopsin in complex with G-protein-coupled receptor kinase 1.
Chen Q, Plasencia M, Li Z, Mukherjee S, Patra D, Chen CL, Klose T, Yao XQ, Kossiakoff AA, Chang L, Andrews PC, Tesmer JJG
Nature 595 600-605 (2021)
PMID: 34262173
Related structures: EMD-23980

Function and Biology Details

Reaction catalysed: 
ATP + [rhodopsin] = ADP + [rhodopsin] phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-208777 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Rhodopsin kinase GRK1 Chain: G
Molecule details ›
Chain: G
Length: 543 amino acids
Theoretical weight: 61.54 KDa
Source organism: Bos taurus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P28327 (Residues: 1-535; Coverage: 95%)
Gene names: GRK1, RHOK
Sequence domains:
Fab6 heavy chain Chain: H
Molecule details ›
Chain: H
Length: 234 amino acids
Theoretical weight: 25.17 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Fab6 light chain Chain: L
Molecule details ›
Chain: L
Length: 216 amino acids
Theoretical weight: 23.51 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
Rhodopsin Chain: R
Molecule details ›
Chain: R
Length: 348 amino acids
Theoretical weight: 39.03 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P02699 (Residues: 1-348; Coverage: 100%)
Gene name: RHO
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand SGV 1 x SGV
Ligand RET 1 x RET
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 4Å
Relevant EMDB volumes: EMD-23980
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli

Quick links

Citations

12 review citations

G protein-coupled receptor signaling: transducers and effectors.
Jiang et al. (2022)
11 more

4 mentions without citation

New insights into GPCR coupling and dimerisation from cryo-EM structures.
Gusach et al. (2023)
3 more