› 7msm

Electron Microscopy
2.79Å resolution

Mtb 70SIC in complex with MtbEttA at Trans_R0 state

Released:
DOI: 10.2210/pdb7msm/pdb
PDB entry 7msm coloured by chain, front view.
PDB entry 7msm coloured by chain, side view.
PDB entry 7msm coloured by chain, top view.
Source organism: Mycobacterium tuberculosis H37Rv
Primary publication:
Interplay between an ATP-binding cassette F protein and the ribosome from Mycobacterium tuberculosis.
Cui Z, Li X, Shin J, Gamper H, Hou YM, Sacchettini JC, Zhang J
Nat Commun 13 432 (2022)
PMID: 35064151
Related structures: EMD-23969

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero 55-mer (preferred)
PDBe Complex ID:
PDB-CPX-235512 (preferred)
Entry contents:
50 distinct polypeptide molecules
5 distinct RNA molecules
Macromolecules (55 distinct):
Large ribosomal subunit protein bL32 Chain: 0
Molecule details ›
Chain: 0
Length: 57 amino acids
Theoretical weight: 6.53 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH99 (Residues: 1-57; Coverage: 100%)
Gene names: Rv0979.1c, Rv0979A, rpmF
Sequence domains: Ribosomal L32p protein family
Large ribosomal subunit protein bL33B Chain: 1
Molecule details ›
Chain: 1
Length: 55 amino acids
Theoretical weight: 6.51 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH95 (Residues: 1-55; Coverage: 100%)
Gene names: MTCY20H10.15A, Rv0634B, rpmG2
Sequence domains: Ribosomal protein L33
Large ribosomal subunit protein bL34 Chain: 2
Molecule details ›
Chain: 2
Length: 47 amino acids
Theoretical weight: 5.64 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH93 (Residues: 1-47; Coverage: 100%)
Gene names: MTV028.15, Rv3924c, rpmH
Sequence domains: Ribosomal protein L34
Large ribosomal subunit protein bL35 Chain: 3
Molecule details ›
Chain: 3
Length: 64 amino acids
Theoretical weight: 7.24 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH91 (Residues: 1-64; Coverage: 100%)
Gene names: MTCY06H11.06, Rv1642, rpmI
Sequence domains: Ribosomal protein L35
Large ribosomal subunit protein bL36 Chain: 4
Molecule details ›
Chain: 4
Length: 37 amino acids
Theoretical weight: 4.32 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH89 (Residues: 1-37; Coverage: 100%)
Gene names: MTCY13E12.14c, Rv3461c, rpmJ
Sequence domains: Ribosomal protein L36
Large ribosomal subunit protein bL31 Chain: 6
Molecule details ›
Chain: 6
Length: 80 amino acids
Theoretical weight: 8.77 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WHA1 (Residues: 1-80; Coverage: 100%)
Gene names: MTCY373.18, Rv1298, rpmE
Sequence domains: Ribosomal protein L31
50S ribosomal protein L37 Chain: 7
Molecule details ›
Chain: 7
Length: 24 amino acids
Theoretical weight: 2.88 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: A0A0U0UFA0 (Residues: 6-29; Coverage: 83%)
Gene names: ERS007703_02732, ERS007739_03374
Large ribosomal subunit protein uL1 Chain: 8
Molecule details ›
Chain: 8
Length: 235 amino acids
Theoretical weight: 24.79 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WHC7 (Residues: 1-235; Coverage: 100%)
Gene names: MTCY20H10.22, Rv0641, rplA
Sequence domains: Ribosomal protein L1p/L10e family
Large ribosomal subunit protein uL2 Chain: C
Molecule details ›
Chain: C
Length: 280 amino acids
Theoretical weight: 30.64 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WHA5 (Residues: 1-280; Coverage: 100%)
Gene names: MTCY210.23, Rv0704, rplB
Sequence domains:
Large ribosomal subunit protein uL3 Chain: D
Molecule details ›
Chain: D
Length: 217 amino acids
Theoretical weight: 23.13 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH87 (Residues: 1-217; Coverage: 100%)
Gene names: MTCY210.20, Rv0701, rplC
Sequence domains: Ribosomal protein L3
Large ribosomal subunit protein uL4 Chain: E
Molecule details ›
Chain: E
Length: 223 amino acids
Theoretical weight: 23.78 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH85 (Residues: 1-223; Coverage: 100%)
Gene names: MTCY210.21, Rv0702, rplD
Sequence domains: Ribosomal protein L4/L1 family
Large ribosomal subunit protein uL5 Chain: F
Molecule details ›
Chain: F
Length: 187 amino acids
Theoretical weight: 21.05 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH83 (Residues: 1-187; Coverage: 100%)
Gene names: MTCY210.35, Rv0716, rplE
Sequence domains:
Large ribosomal subunit protein uL6 Chain: G
Molecule details ›
Chain: G
Length: 179 amino acids
Theoretical weight: 19.41 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH81 (Residues: 1-179; Coverage: 100%)
Gene names: MTCY210.38, Rv0719, rplF
Sequence domains: Ribosomal protein L6
Large ribosomal subunit protein bL9 Chain: H
Molecule details ›
Chain: H
Length: 152 amino acids
Theoretical weight: 16.2 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH79 (Residues: 1-152; Coverage: 100%)
Gene names: MTCY21D4.19, Rv0056, rplI
Sequence domains:
Large ribosomal subunit protein uL13 Chain: J
Molecule details ›
Chain: J
Length: 195 amino acids
Theoretical weight: 21.65 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: A0A0T9D5H2 (Residues: 1-195; Coverage: 100%)
Gene names: ERS007657_03631, ERS007661_03121, ERS007679_01993, ERS007681_03424, ERS007703_03116, ERS027646_01625, ERS027659_01778, ERS027661_01576, rplM
Sequence domains: Ribosomal protein L13
Large ribosomal subunit protein uL14 Chain: K
Molecule details ›
Chain: K
Length: 122 amino acids
Theoretical weight: 13.42 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WHD9 (Residues: 1-122; Coverage: 100%)
Gene names: MTCY210.33, Rv0714, rplN
Sequence domains: Ribosomal protein L14p/L23e
Large ribosomal subunit protein uL15 Chain: L
Molecule details ›
Chain: L
Length: 146 amino acids
Theoretical weight: 15.57 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WHD7 (Residues: 1-146; Coverage: 100%)
Gene names: MTCY210.42, Rv0723, rplO
Sequence domains: Ribosomal proteins 50S-L15, 50S-L18e, 60S-L27A
Large ribosomal subunit protein uL16 Chain: M
Molecule details ›
Chain: M
Length: 138 amino acids
Theoretical weight: 15.72 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WHD5 (Residues: 1-138; Coverage: 100%)
Gene names: MTCY210.27, Rv0708, rplP
Sequence domains: Ribosomal protein L16p/L10e
Large ribosomal subunit protein bL17 Chain: N
Molecule details ›
Chain: N
Length: 180 amino acids
Theoretical weight: 19.51 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WHD3 (Residues: 1-180; Coverage: 100%)
Gene names: MTCY13E12.09c, Rv3456c, rplQ
Sequence domains: Ribosomal protein L17
Large ribosomal subunit protein uL18 Chain: O
Molecule details ›
Chain: O
Length: 122 amino acids
Theoretical weight: 13.21 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WHD1 (Residues: 1-122; Coverage: 100%)
Gene names: MTCY210.39, Rv0720, rplR
Sequence domains: Ribosomal L18 of archaea, bacteria, mitoch. and chloroplast
Large ribosomal subunit protein bL19 Chain: P
Molecule details ›
Chain: P
Length: 113 amino acids
Theoretical weight: 13.04 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WHC9 (Residues: 1-113; Coverage: 100%)
Gene names: MTCY274.35c, Rv2904c, rplS
Sequence domains: Ribosomal protein L19
Large ribosomal subunit protein bL20 Chain: Q
Molecule details ›
Chain: Q
Length: 129 amino acids
Theoretical weight: 14.56 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WHC5 (Residues: 1-129; Coverage: 100%)
Gene names: MTCY06H11.07, Rv1643, rplT
Sequence domains: Ribosomal protein L20
Large ribosomal subunit protein bL21 Chain: R
Molecule details ›
Chain: R
Length: 104 amino acids
Theoretical weight: 11.17 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WHC3 (Residues: 1-103; Coverage: 100%)
Gene names: MTCY428.04, Rv2442c, rplU
Sequence domains: Ribosomal prokaryotic L21 protein
Large ribosomal subunit protein uL22 Chain: S
Molecule details ›
Chain: S
Length: 197 amino acids
Theoretical weight: 20.42 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WHC1 (Residues: 1-197; Coverage: 100%)
Gene names: MTCY210.25, Rv0706, rplV
Sequence domains: Ribosomal protein L22p/L17e
Large ribosomal subunit protein uL23 Chain: T
Molecule details ›
Chain: T
Length: 100 amino acids
Theoretical weight: 10.98 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WHB9 (Residues: 1-100; Coverage: 100%)
Gene names: MTCY210.22, Rv0703, rplW
Sequence domains: Ribosomal protein L23
Large ribosomal subunit protein uL24 Chain: U
Molecule details ›
Chain: U
Length: 105 amino acids
Theoretical weight: 11.5 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WHB7 (Residues: 1-105; Coverage: 100%)
Gene names: MTCY210.34, Rv0715, rplX
Sequence domains:
Large ribosomal subunit protein bL25 Chain: V
Molecule details ›
Chain: V
Length: 215 amino acids
Theoretical weight: 22.47 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WHB5 (Residues: 1-215; Coverage: 100%)
Gene names: MTCY10G2.34, Rv1015c, ctc, rplY
Sequence domains:
Large ribosomal subunit protein bL27 Chain: W
Molecule details ›
Chain: W
Length: 86 amino acids
Theoretical weight: 8.99 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WHB3 (Residues: 1-86; Coverage: 100%)
Gene names: MTCY428.05, Rv2441c, rpmA
Sequence domains: Ribosomal L27 protein
Large ribosomal subunit protein bL28C Chain: X
Molecule details ›
Chain: X
Length: 64 amino acids
Theoretical weight: 6.92 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P0DV54 (Residues: 1-64; Coverage: 100%)
Gene names: Rv2975A, rpmB3
Sequence domains: Ribosomal L28 family
Large ribosomal subunit protein uL29 Chain: Y
Molecule details ›
Chain: Y
Length: 77 amino acids
Theoretical weight: 8.87 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WHA7 (Residues: 1-77; Coverage: 100%)
Gene names: MTCY210.28, Rv0709, rpmC
Sequence domains: Ribosomal L29 protein
Large ribosomal subunit protein uL30 Chain: Z
Molecule details ›
Chain: Z
Length: 65 amino acids
Theoretical weight: 7.36 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WHA3 (Residues: 1-65; Coverage: 100%)
Gene names: MTCY210.41, Rv0722, rpmD
Sequence domains: Ribosomal protein L30p/L7e
Small ribosomal subunit protein uS3 Chain: c
Molecule details ›
Chain: c
Length: 274 amino acids
Theoretical weight: 30.07 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH37 (Residues: 1-274; Coverage: 100%)
Gene names: MTCY210.26, Rv0707, rpsC
Sequence domains:
Small ribosomal subunit protein uS4 Chain: d
Molecule details ›
Chain: d
Length: 201 amino acids
Theoretical weight: 23.52 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH35 (Residues: 1-201; Coverage: 100%)
Gene names: MTCY13E12.11c, Rv3458c, rpsD
Sequence domains:
Small ribosomal subunit protein uS5 Chain: e
Molecule details ›
Chain: e
Length: 220 amino acids
Theoretical weight: 22.93 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH33 (Residues: 1-220; Coverage: 100%)
Gene names: MTCY210.40, Rv0721, rpsE
Sequence domains:
Small ribosomal subunit protein bS6 Chain: f
Molecule details ›
Chain: f
Length: 96 amino acids
Theoretical weight: 10.95 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH31 (Residues: 1-96; Coverage: 100%)
Gene names: MTCY21D4.16, Rv0053, rpsF
Sequence domains: Ribosomal protein S6
Small ribosomal subunit protein uS7 Chain: g
Molecule details ›
Chain: g
Length: 156 amino acids
Theoretical weight: 17.63 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH29 (Residues: 1-156; Coverage: 100%)
Gene names: MTV040.11, Rv0683, rps7, rpsG
Sequence domains: Ribosomal protein S7p/S5e
Small ribosomal subunit protein uS8 Chain: h
Molecule details ›
Chain: h
Length: 132 amino acids
Theoretical weight: 14.43 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH27 (Residues: 1-132; Coverage: 100%)
Gene names: MTCY210.37, Rv0718, rpsH
Sequence domains: Ribosomal protein S8
Small ribosomal subunit protein uS9 Chain: i
Molecule details ›
Chain: i
Length: 151 amino acids
Theoretical weight: 16.47 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH25 (Residues: 1-151; Coverage: 100%)
Gene names: MTCY77.14c, Rv3442c, rpsI
Sequence domains: Ribosomal protein S9/S16
Small ribosomal subunit protein uS10 Chain: j
Molecule details ›
Chain: j
Length: 101 amino acids
Theoretical weight: 11.45 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH67 (Residues: 1-101; Coverage: 100%)
Gene names: MTCY210.19, Rv0700, rpsJ, rpsX
Sequence domains: Ribosomal protein S10p/S20e
Small ribosomal subunit protein uS11 Chain: k
Molecule details ›
Chain: k
Length: 139 amino acids
Theoretical weight: 14.82 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH65 (Residues: 1-139; Coverage: 100%)
Gene names: MTCY13E12.12c, Rv3459c, rpsK
Sequence domains: Ribosomal protein S11
Small ribosomal subunit protein uS12 Chain: l
Molecule details ›
Chain: l
Length: 124 amino acids
Theoretical weight: 13.88 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH63 (Residues: 1-124; Coverage: 100%)
Gene names: MTV040.10, Rv0682, rps12, rpsL
Sequence domains: Ribosomal protein S12/S23
Small ribosomal subunit protein uS13 Chain: m
Molecule details ›
Chain: m
Length: 124 amino acids
Theoretical weight: 14.38 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH61 (Residues: 1-124; Coverage: 100%)
Gene names: MTCY13E12.13c, Rv3460c, rpsM
Sequence domains: Ribosomal protein S13/S18
Small ribosomal subunit protein uS14B Chain: n
Molecule details ›
Chain: n
Length: 61 amino acids
Theoretical weight: 6.84 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH57 (Residues: 1-61; Coverage: 100%)
Gene names: MTCY210.36, Rv0717, rpsN1, rpsZ
Sequence domains: Ribosomal protein S14p/S29e
Small ribosomal subunit protein uS15 Chain: o
Molecule details ›
Chain: o
Length: 89 amino acids
Theoretical weight: 10.5 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH55 (Residues: 1-89; Coverage: 100%)
Gene names: MTV002.50c, Rv2785c, rpsO
Sequence domains: Ribosomal protein S15
Small ribosomal subunit protein bS16 Chain: p
Molecule details ›
Chain: p
Length: 162 amino acids
Theoretical weight: 17.47 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH53 (Residues: 1-162; Coverage: 100%)
Gene names: MTCY274.41c, Rv2909c, rpsP
Sequence domains: Ribosomal protein S16
Small ribosomal subunit protein uS17 Chain: q
Molecule details ›
Chain: q
Length: 135 amino acids
Theoretical weight: 14.78 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH51 (Residues: 1-135; Coverage: 100%)
Gene names: MTCY210.29, Rv0710, rpsQ
Sequence domains: Ribosomal protein S17
Small ribosomal subunit protein bS18A Chain: r
Molecule details ›
Chain: r
Length: 84 amino acids
Theoretical weight: 9.57 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH49 (Residues: 1-84; Coverage: 100%)
Gene names: MTCY21D4.18, Rv0055, rpsR, rpsR1
Sequence domains: Ribosomal protein S18
Small ribosomal subunit protein uS19 Chain: s
Molecule details ›
Chain: s
Length: 93 amino acids
Theoretical weight: 10.83 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH45 (Residues: 1-93; Coverage: 100%)
Gene names: MTCY210.24, Rv0705, rpsS
Sequence domains: Ribosomal protein S19
Small ribosomal subunit protein bS20 Chain: t
Molecule details ›
Chain: t
Length: 86 amino acids
Theoretical weight: 9.43 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WH41 (Residues: 1-86; Coverage: 100%)
Gene names: MTCY253.08c, Rv2412, rpsT
Sequence domains: Ribosomal protein S20
Energy-dependent translational throttle protein EttA Chain: x
Molecule details ›
Chain: x
Length: 558 amino acids
Theoretical weight: 61.97 KDa
Source organism: Mycobacterium tuberculosis H37Rv
UniProt:
  • Canonical: P9WQK3 (Residues: 1-558; Coverage: 100%)
Gene names: Rv2477c, ettA
Sequence domains:
23S ribosomal RNA Chain: A
Molecule details ›
Chain: A
Length: 3138 nucleotides
Theoretical weight: 1017.82 KDa
Sequence domains: Bacterial large subunit ribosomal RNA
5S ribosomal RNA Chain: B
Molecule details ›
Chain: B
Length: 115 nucleotides
Theoretical weight: 37.1 KDa
Sequence domains: 5S ribosomal RNA
16S ribosomal RNA Chain: a
Molecule details ›
Chain: a
Length: 1537 nucleotides
Theoretical weight: 498.4 KDa
Sequence domains: Bacterial small subunit ribosomal RNA
initiator tRNA (Met) Chain: y
Molecule details ›
Chain: y
Length: 77 nucleotides
Theoretical weight: 24.8 KDa
Sequence domains: tRNA
Messenger RNA Chain: z
Molecule details ›
Chain: z
Length: 26 nucleotides
Theoretical weight: 8.47 KDa

Ligands and Environments

5 bound ligands:
Ligand ZN 6 x ZN
Ligand MG 445 x MG
Ligand ADP 2 x ADP
Ligand VO4 2 x VO4
Ligand FME 1 x FME
8 modified residues:
Ligand 5MU 1 x 5MU
Ligand OMG 1 x OMG
Ligand G7M 1 x G7M
Ligand 2MG 1 x 2MG
Ligand 5MC 1 x 5MC
Ligand 4OC 1 x 4OC
Ligand UR3 1 x UR3
Ligand MA6 2 x MA6

Experiments and Validation Details

Entry percentile scores
Resolution: 2.79Å
Relevant EMDB volumes: EMD-23969

Quick links

Citations

1 review citation

Unraveling the mechanisms of intrinsic drug resistance in Mycobacterium tuberculosis.
Poulton et al. (2022)
5 other articles

1 mention without citation

Interaction of Vanadium Complexes with Proteins: Revisiting the Reported Structures in the Protein Data Bank (PDB) since 2015.
Santos et al. (2023)