› 7mov

X-ray diffraction
1.65Å resolution

PTP1B 1-301 F225Y-R199N mutations

Released:
DOI: 10.2210/pdb7mov/pdb
PDB entry 7mov coloured by chain, front view.
PDB entry 7mov coloured by chain, side view.
PDB entry 7mov coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Conserved conformational dynamics determine enzyme activity.
Torgeson KR, Clarkson MW, Granata D, Lindorff-Larsen K, Page R, Peti W
Sci Adv 8 eabo5546 (2022)
PMID: 35921420

Function and Biology Details

Reaction catalysed: 
Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Tyrosine-protein phosphatase non-receptor type 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 308 amino acids
Theoretical weight: 35.77 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P18031 (Residues: 1-301; Coverage: 69%)
Gene names: PTP1B, PTPN1
Sequence domains: Protein-tyrosine phosphatase

Ligands and Environments

3 bound ligands:
Ligand GOL 10 x GOL
Ligand CL 3 x CL
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL12-2
Spacegroup: C2
Unit cell:
a: 114.359Å b: 90.102Å c: 74.49Å
α: 90° β: 110.933° γ: 90°
R-values:
R R work R free
0.167 0.165 0.193
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Loop dynamics and the evolution of enzyme activity.
Corbella et al. (2023)
9 other articles

1 mention without citation

Native dynamics and allosteric responses in PTP1B probed by high-resolution HDX-MS.
Woods et al. (2023)