› 7mjr

X-ray diffraction
3.22Å resolution

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104534 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iota toxin protein Ib Chain: A
Molecule details ›
Chain: A
Length: 937 amino acids
Theoretical weight: 105.18 KDa
Source organism: Bacillus thuringiensis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A2U5FTM5 (Residues: 1-937; Coverage: 100%)
Gene name: vip4Da1
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand CA 3 x CA
Ligand SO4 9 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2221
Unit cell:
a: 117.517Å b: 221.374Å c: 154.141Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.196 0.241
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 citation in other articles

Chromobacterium Csp_P biopesticide is toxic to larvae of three Diabrotica species including strains resistant to Bacillus thuringiensis.
Pereira et al. (2022)
1 more

1 mention without citation

Structural and functional insights into the first Bacillus thuringiensis vegetative insecticidal protein of the Vpb4 fold, active against western corn rootworm.
Kouadio et al. (2021)