› 7mha

X-ray diffraction
2.79Å resolution

Crystal structure of R. sphaeroides Photosynthetic Reaction Center variant; W252V mutant

Released:
DOI: 10.2210/pdb7mha/pdb
PDB 7mha coloured by chain and viewed from the front.
PDB 7mha coloured by chain and viewed from the side.
PDB 7mha coloured by chain and viewed from the top.
Source organism: Cereibacter sphaeroides
Primary publication:
Photosynthetic reaction center variants made via genetic code expansion indicate tyrosine at M210 tunes the mechanism for primary electron transfer
Weaver JB, Lin CY, Faries KM, Mathews II, Russi S, Holten D, Kirmaier C, Boxer SG
Thesis Ph.D. Stanford University 294-294 (2022)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Reaction center protein H chain Chain: H
Molecule details ›
Chain: H
Length: 266 amino acids
Theoretical weight: 28.92 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: Q3J170 (Residues: 1-259; Coverage: 100%)
Gene names: RHOS4_18960, RSP_0291, puhA
Sequence domains:
Reaction center protein L chain Chain: L
Molecule details ›
Chain: L
Length: 282 amino acids
Theoretical weight: 31.48 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: Q3J1A5 (Residues: 1-282; Coverage: 100%)
Gene names: RHOS4_18610, RSP_0257, pufL
Sequence domains: Photosynthetic reaction centre protein
Reaction center protein M chain Chain: M
Molecule details ›
Chain: M
Length: 308 amino acids
Theoretical weight: 34.44 KDa
Source organism: Cereibacter sphaeroides
Expression system: Cereibacter sphaeroides
UniProt:
  • Canonical: Q3J1A6 (Residues: 1-308; Coverage: 100%)
Gene names: RHOS4_18600, RSP_0256, pufM
Sequence domains: Photosynthetic reaction centre protein

Ligands and Environments

8 bound ligands:
Ligand BCL 4 x BCL
Ligand BPH 2 x BPH
Ligand U10 1 x U10
Ligand CL 1 x CL
Ligand LDA 3 x LDA
Ligand FE 1 x FE
Ligand SPO 1 x SPO
Ligand CDL 1 x CDL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL14-1
Spacegroup: P3121
Unit cell:
a: 142.193Å b: 142.193Å c: 187.673Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.157 0.156 0.178
Expression system: Cereibacter sphaeroides

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