› 7m99

Electron Microscopy
3.2Å resolution

ATPgS bound TnsC filament from ShCAST system

Released:
DOI: 10.2210/pdb7m99/pdb
PDB entry 7m99 coloured by chain, front view.
PDB entry 7m99 coloured by chain, side view.
PDB entry 7m99 coloured by chain, top view.
Source organisms:
Primary publication:
Structural basis for target site selection in RNA-guided DNA transposition systems.
Park JU, Tsai AW, Mehrotra E, Petassi MT, Hsieh SC, Ke A, Peters JE, Kellogg EH
Science 373 768-774 (2021)
PMID: 34385391
Related structures: EMD-23720

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero decamer (preferred)
PDBe Complex ID:
PDB-CPX-121616 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
TnsC Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 276 amino acids
Theoretical weight: 31.44 KDa
Source organism: Scytonema hofmannii
Expression system: Escherichia coli BL21
DNA (5'-D(P*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*TP*T)-3') Chain: I
Molecule details ›
Chain: I
Length: 21 nucleotides
Theoretical weight: 6.34 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*AP*A)-3') Chain: J
Molecule details ›
Chain: J
Length: 21 nucleotides
Theoretical weight: 6.53 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand AGS 7 x AGS
Ligand MG 7 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.2Å
Relevant EMDB volumes: EMD-23720
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

6 review citations

CRISPR-based genome editing through the lens of DNA repair.
Nambiar et al. (2022)
5 more

3 mentions without citation

Structural basis for target site selection in RNA-guided DNA transposition systems.
Park et al. (2021)
2 more