› 7lrv

X-ray diffraction
1.4Å resolution

Ni-bound crystal structure of the engineered cyt cb562 variant, DiCyt2, crystallized in the presence of Ni(II)

Released:
DOI: 10.2210/pdb7lrv/pdb
PDB entry 7lrv coloured by chain, front view.
PDB entry 7lrv coloured by chain, side view.
PDB entry 7lrv coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Overcoming universal restrictions on metal selectivity by protein design.
Choi TS, Tezcan FA
Nature 603 522-527 (2022)
PMID: 35236987

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142156 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Soluble cytochrome b562 Chains: A, C
Molecule details ›
Chains: A, C
Length: 106 amino acids
Theoretical weight: 11.92 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ABE7 (Residues: 23-128; Coverage: 100%)
Gene name: cybC
Sequence domains: Cytochrome b562

Ligands and Environments

2 bound ligands:
Ligand HEC 2 x HEC
Ligand NI 2 x NI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P212121
Unit cell:
a: 54.91Å b: 60.72Å c: 63.77Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.19 0.216
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Small-angle X-ray scattering studies of enzymes.
Byer et al. (2023)
1 more

1 mention without citation

Overcoming universal restrictions on metal selectivity by protein design.
Choi et al. (2022)