7lru

X-ray diffraction
1.6Å resolution

Crystal structure of SFPQ-NONO-SFPQ chimeric protein homodimer

Released:
Source organism: Homo sapiens
Entry authors: Marshall AC, Bond CS, Mohnen I, Knott GJ

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Non-POU domain-containing octamer-binding protein; Splicing factor, proline- and glutamine-rich Chain: B
Molecule details ›
Chain: B
Length: 263 amino acids
Theoretical weight: 30.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23246 (Residues: 278-456, 505-535; Coverage: 30%)
  • Canonical: Q15233 (Residues: 53-54, 234-281; Coverage: 11%)
Gene names: NONO, NRB54, PSF, SFPQ
Sequence domains: RNA recognition motif. (a.k.a. RRM, RBD, or RNP domain)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: C2221
Unit cell:
a: 73.665Å b: 107.162Å c: 68.305Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.218 0.256
Expression system: Escherichia coli BL21(DE3)