› 7lqx

X-ray diffraction
1.3Å resolution

Crystal structure of a GH5_18 from Bifidobacterium longum subsp. infantis

Released:
DOI: 10.2210/pdb7lqx/pdb
PDB entry 7lqx coloured by chain, front view.
PDB entry 7lqx coloured by chain, side view.
PDB entry 7lqx coloured by chain, top view.
Source organism: Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088
Primary publication:
N-Glycan Degradation Pathways in Gut- and Soil-Dwelling Actinobacteria Share Common Core Genes.
Higgins MA, Tegl G, MacDonald SS, Arnal G, Brumer H, Withers SG, Ryan KS
ACS Chem Biol 16 701-711 (2021)
PMID: 33764747

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-110474 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 51.46 KDa
Source organism: Bifidobacterium longum subsp. infantis ATCC 15697 = JCM 1222 = DSM 20088
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B7GNS6 (Residues: 1-431; Coverage: 100%)
Gene name: Blon_2377

Ligands and Environments

2 bound ligands:
Ligand EDO 4 x EDO
Ligand SO4 3 x SO4
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: C2
Unit cell:
a: 88.83Å b: 51.72Å c: 85.45Å
α: 90° β: 91.24° γ: 90°
R-values:
R R work R free
0.137 0.136 0.152
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Carbohydrate-active enzymes (CAZymes) in the gut microbiome.
Wardman et al. (2022)
1 more