7lq0

X-ray diffraction
1.6Å resolution

Crystal structures and ribonuclease activity of the Flavivirus host factor ERI3 that is involved in viral RNA synthesis define the ERI subfamily of structure-specific 3-prime - 5-prime exoribonucleases

Released:
Source organism: Homo sapiens
Entry authors: Thapar R, Andrew AS, Tainer JA

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ERI1 exoribonuclease 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 216 amino acids
Theoretical weight: 25.07 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O43414 (Residues: 137-337; Coverage: 60%)
  • Best match: O43414-3 (Residues: 1-128)
Gene names: ERI3, PINT1, PRNPIP, PRNPIP1
Sequence domains: Exonuclease

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P212121
Unit cell:
a: 58.529Å b: 74.2Å c: 96.487Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.164 0.162 0.199
Expression system: Escherichia coli