› 7lnw

X-ray diffraction
2.29Å resolution

I146A mutant of the isopentenyl phosphate kinase from Candidatus methanomethylophilus alvus

Released:
DOI: 10.2210/pdb7lnw/pdb
PDB entry 7lnw coloured by chain, front view.
PDB entry 7lnw coloured by chain, side view.
PDB entry 7lnw coloured by chain, top view.
Source organism: Candidatus Methanomethylophilus alvus
Primary publication:
Johnson BP, Kumar V, Scull EM, Thomas LM, Bourne CR, Singh S
ACS Chem Biol 17 85-102 (2022)
PMID: 34905349

Function and Biology Details

Reaction catalysed: 
ATP + 3-methylbut-3-en-1-yl phosphate = ADP + 3-methylbut-3-en-1-yl diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-126073 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isopentenyl phosphate kinase Chains: A, B
Molecule details ›
Chains: A, B
Length: 279 amino acids
Theoretical weight: 30.49 KDa
Source organism: Candidatus Methanomethylophilus alvus
Expression system: Escherichia coli
UniProt:
  • Canonical: M9SEQ1 (Residues: 1-259; Coverage: 100%)
Gene name: MMALV_14490
Sequence domains: Amino acid kinase family

Ligands and Environments

2 bound ligands:
Ligand ADP 2 x ADP
Ligand Y77 2 x Y77
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 49.249Å b: 92.998Å c: 52.145Å
α: 90° β: 92.017° γ: 90°
R-values:
R R work R free
0.209 0.204 0.256
Expression system: Escherichia coli

Quick links

Citations

1 mention without citation

Molecular Basis for the Substrate Promiscuity of Isopentenyl Phosphate Kinase from Candidatus methanomethylophilus alvus.
Johnson et al. (2022)