› 7lgp

X-ray diffraction
1.91Å resolution

DapE enzyme from Shigella flexneri

Released:
DOI: 10.2210/pdb7lgp/pdb
PDB entry 7lgp coloured by chain, front view.
PDB entry 7lgp coloured by chain, side view.
PDB entry 7lgp coloured by chain, top view.
Source organism: Shigella flexneri 2a str. 301
Entry authors: Osipiuk J, Endres M, Becker DP, Joachimiak A, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed: 
N-succinyl-LL-2,6-diaminoheptanedioate + H(2)O = succinate + LL-2,6-diaminoheptanedioate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142435 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Succinyl-diaminopimelate desuccinylase Chains: A, B
Molecule details ›
Chains: A, B
Length: 378 amino acids
Theoretical weight: 41.58 KDa
Source organism: Shigella flexneri 2a str. 301
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AED8 (Residues: 1-375; Coverage: 100%)
Gene names: S2665, SF2514, dapE
Sequence domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand CL 15 x CL
Ligand NA 3 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: R32
Unit cell:
a: 180.141Å b: 180.141Å c: 200.091Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.16 0.159 0.187
Expression system: Escherichia coli BL21(DE3)

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