X-ray diffraction
1.92Å resolution

Crystal structure of aminoglycoside acetyltransferase AAC(3)-IIb

Source organism: Serratia marcescens
Entry authors: Stogios PJ, Evdokimova E, Osipiuk J, Di Leo R, Savchenko A, Joachimiak A, Satchell KJF, Center for Structural Genomics of Infectious Diseases (CSGID)

Function and Biology Details

Reaction catalysed:
Acetyl-CoA + a 2-deoxystreptamine antibiotic = CoA + N(3)-acetyl-2-deoxystreptamine antibiotic
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Aminoglycoside N(3)-acetyltransferase III Chain: A
Molecule details ›
Chain: A
Length: 271 amino acids
Theoretical weight: 29.02 KDa
Source organism: Serratia marcescens
Expression system: Escherichia coli
  • Canonical: Q01515 (Residues: 1-269; Coverage: 100%)
Gene name: aac3-Vb
Sequence domains: Aminoglycoside 3-N-acetyltransferase

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 43.174Å b: 61.434Å c: 112.031Å
α: 90° β: 90° γ: 90°
R R work R free
0.182 0.18 0.229
Expression system: Escherichia coli