7l3l

X-ray diffraction
2.8Å resolution

Structure of TRAF5 and TRAF6 RING Hetero dimer

Released:

Function and Biology Details

Reaction catalysed:
S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
TNF receptor-associated factor 5 Chains: A, C
Molecule details ›
Chains: A, C
Length: 142 amino acids
Theoretical weight: 16.59 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O00463 (Residues: 23-164; Coverage: 26%)
Gene names: RNF84, TRAF5
Sequence domains: Zinc finger, C3HC4 type (RING finger)
TNF receptor-associated factor 6 Chains: B, D
Molecule details ›
Chains: B, D
Length: 107 amino acids
Theoretical weight: 12.33 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y4K3 (Residues: 52-158; Coverage: 21%)
Gene names: RNF85, TRAF6
Sequence domains: Zinc finger, C3HC4 type (RING finger)

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Spacegroup: P21
Unit cell:
a: 41.597Å b: 106.062Å c: 94.427Å
α: 90° β: 102.64° γ: 90°
R-values:
R R work R free
0.21 0.204 0.234
Expression system: Escherichia coli