7l32

X-ray diffraction
1.9Å resolution

Molybdopterin biosynthesis MoaE protein from Burkholderia ambifaria MC40-6

Released:
Entry author: Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed:
Cyclic pyranopterin phosphate + 2 [molybdopterin-synthase sulfur-carrier protein]-Gly-NH-CH(2)-C(O)SH + H(2)O = molybdopterin + 2 [molybdopterin-synthase sulfur-carrier protein]
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Molybdopterin synthase catalytic subunit Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 166 amino acids
Theoretical weight: 18.42 KDa
Source organism: Burkholderia ambifaria MC40-6
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B1YRH1 (Residues: 1-158; Coverage: 100%)
Gene name: BamMC406_1760
Sequence domains: MoaE protein

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: C2
Unit cell:
a: 111.68Å b: 93.29Å c: 65.25Å
α: 90° β: 90.103° γ: 90°
R-values:
R R work R free
0.18 0.179 0.203
Expression system: Escherichia coli BL21(DE3)