› 7l1z

X-ray diffraction
2.05Å resolution

Unlocking the structural features for the exo-xylobiosidase activity of an unusual GH11 member identified in a compost-derived consortium - NT-truncated form

Released:
DOI: 10.2210/pdb7l1z/pdb
PDB entry 7l1z coloured by chain, front view.
PDB entry 7l1z coloured by chain, side view.
PDB entry 7l1z coloured by chain, top view.
Source organism: unidentified
Primary publication:
Unlocking the structural features for the xylobiohydrolase activity of an unusual GH11 member identified in a compost-derived consortium.
Kadowaki MAS, Briganti L, Evangelista DE, Echevarría-Poza A, Tryfona T, Pellegrini VOA, Nakayama DG, Dupree P, Polikarpov I
Biotechnol Bioeng 118 4052-4064 (2021)
PMID: 34232504

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-104238 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
endo-1,4-beta-xylanase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 221 amino acids
Theoretical weight: 24.89 KDa
Source organism: unidentified
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A2H4TGG5 (Residues: 49-268; Coverage: 89%)
Gene name: xyn11a
Sequence domains: Glycosyl hydrolases family 11

Ligands and Environments

2 bound ligands:
Ligand EDO 5 x EDO
Ligand SO4 15 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: LNLS BEAMLINE W01B-MX2
Spacegroup: C2221
Unit cell:
a: 132.562Å b: 149.686Å c: 79.615Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.226
Expression system: Escherichia coli BL21(DE3)

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