› 7l1r

Electron Microscopy
3.1Å resolution

PS3 F1-ATPase Hydrolysis Dwell

Released:
DOI: 10.2210/pdb7l1r/pdb
PDB entry 7l1r coloured by chain, front view.
PDB entry 7l1r coloured by chain, side view.
PDB entry 7l1r coloured by chain, top view.
Source organism: Bacillus sp. PS3
Primary publication:
The six steps of the complete F1-ATPase rotary catalytic cycle.
Sobti M, Ueno H, Noji H, Stewart AG
Nat Commun 12 4690 (2021)
PMID: 34344897
Related structures: EMD-23116

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-102508 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
ATP synthase subunit alpha Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 510 amino acids
Theoretical weight: 55.88 KDa
Source organism: Bacillus sp. PS3
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0M3VGF9 (Residues: 2-502; Coverage: 100%)
Gene names: atpA, uncA
Sequence domains:
ATP synthase subunit beta Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 484 amino acids
Theoretical weight: 53.41 KDa
Source organism: Bacillus sp. PS3
Expression system: Escherichia coli
UniProt:
  • Canonical: P07677 (Residues: 1-473; Coverage: 100%)
Gene name: atpD
Sequence domains:
ATP synthase gamma chain Chain: G
Molecule details ›
Chain: G
Length: 285 amino acids
Theoretical weight: 31.87 KDa
Source organism: Bacillus sp. PS3
Expression system: Escherichia coli
UniProt:
  • Canonical: A0A0M4TPJ7 (Residues: 1-285; Coverage: 100%)
Gene names: atpG, uncG
Sequence domains: ATP synthase

Ligands and Environments

3 bound ligands:
Ligand ATP 5 x ATP
Ligand MG 6 x MG
Ligand ADP 1 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.1Å
Relevant EMDB volumes: EMD-23116
Expression system: Escherichia coli

Quick links

Citations

7 review citations

How Does F1-ATPase Generate Torque?: Analysis From Cryo-Electron Microscopy and Rotational Catalysis of Thermophilic F1.
Noji et al. (2022)
6 more

2 mentions without citation

CryoEM Reveals the Complexity and Diversity of ATP Synthases.
Courbon et al. (2022)
1 more