› 7l1b

X-ray diffraction
2.04Å resolution

Crystal structure of HLA-A*03:01 in complex with a wild-type PIK3CA peptide

Released:
DOI: 10.2210/pdb7l1b/pdb
PDB entry 7l1b coloured by chain, front view.
PDB entry 7l1b coloured by chain, side view.
PDB entry 7l1b coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Immunogenicity and therapeutic targeting of a public neoantigen derived from mutated PIK3CA.
Chandran SS, Ma J, Klatt MG, Dündar F, Bandlamudi C, Razavi P, Wen HY, Weigelt B, Zumbo P, Fu SN, Banks LB, Yi F, Vercher E, Etxeberria I, Bestman WD, Da Cruz Paula A, Aricescu IS, Drilon A, Betel D, Scheinberg DA, Baker BM, Klebanoff CA
Nat Med 28 946-957 (2022)
PMID: 35484264

Function and Biology Details

Reactions catalysed: 
ATP + 1-phosphatidyl-1D-myo-inositol = ADP + 1-phosphatidyl-1D-myo-inositol 3-phosphate
ATP + 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate = ADP + 1-phosphatidyl-1D-myo-inositol 3,4,5-trisphosphate
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-137691 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
HLA class I histocompatibility antigen, A alpha chain Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 31.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P04439 (Residues: 25-298; Coverage: 80%)
Gene names: HLA-A, HLAA
Sequence domains:
Beta-2-microglobulin Chain: B
Molecule details ›
Chain: B
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit alpha isoform Chain: C
Molecule details ›
Chain: C
Length: 9 amino acids
Theoretical weight: 997 Da
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P42336 (Residues: 1046-1054; Coverage: 1%)
Gene name: PIK3CA

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P622
Unit cell:
a: 156.592Å b: 156.592Å c: 86.213Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.196 0.195 0.221
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

16 review citations

Neoantigens: promising targets for cancer therapy.
Xie et al. (2023)
15 more