X-ray diffraction
1.6Å resolution

Crystal structure of s-formylglutathione hydrolase (FrmB) from Staphylococcus aureus, apoenzyme


Function and Biology Details

Reaction catalysed:
S-formylglutathione + H(2)O = glutathione + formate
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
S-formylglutathione hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 261 amino acids
Theoretical weight: 29.93 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
  • Canonical: Q2FUY3 (Residues: 1-253; Coverage: 100%)
Gene name: SAOUHSC_02962
Sequence domains: Putative esterase

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 128.303Å b: 80.5Å c: 66.651Å
α: 90° β: 113.84° γ: 90°
R R work R free
0.158 0.157 0.177
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'