7kzi

X-ray diffraction
2.82Å resolution

Intermediate state (QQQ) of near full-length DnaK alternatively fused with a substrate peptide

Released:
Source organism: Escherichia coli K-12
Primary publication:
Intermediates in allosteric equilibria of DnaK-ATP interactions with substrate peptides
Acta Crystallogr. D Biol. Crystallogr. 77 606-617 (2021)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Chaperone protein DnaK Chains: A, B
Molecule details ›
Chains: A, B
Length: 628 amino acids
Theoretical weight: 67.93 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: P0A6Y8 (Residues: 1-609; Coverage: 96%)
Gene names: JW0013, b0014, dnaK, groP, grpF, seg
Sequence domains: Hsp70 protein

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P6422
Unit cell:
a: 121.518Å b: 121.518Å c: 457.878Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.256 0.255 0.273
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'