Electron Microscopy
2.08Å resolution

Cryo-EM structure of SARS-CoV-2 ORF3a


Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
Non-polymer only tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
ORF3a protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 284 amino acids
Theoretical weight: 32.17 KDa
Source organism: Severe acute respiratory syndrome coronavirus 2
Expression system: Spodoptera frugiperda
  • Canonical: P0DTC3 (Residues: 1-275; Coverage: 100%)
Gene name: 3a
Sequence domains: Betacoronavirus viroporin
Truncated apolipoprotein A-I Chains: C, D
Molecule details ›
Chains: C, D
Length: 210 amino acids
Theoretical weight: 24.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P02647 (Residues: 79-267; Coverage: 76%)
Gene name: APOA1

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 2.08Å
Relevant EMDB volumes: EMD-22898
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli