› 7kg2

X-ray diffraction
1.89Å resolution

Dihydrodipicolinate synthase (DHDPS) from C.jejuni, H59K mutant with pyruvate bound in the active site and L-histidine bound at the allosteric site

Released:
DOI: 10.2210/pdb7kg2/pdb
PDB entry 7kg2 coloured by chain, front view.
PDB entry 7kg2 coloured by chain, side view.
PDB entry 7kg2 coloured by chain, top view.
Source organism: Campylobacter jejuni
Entry authors: Saran S, Majdi Yazdi M, Sanders DAR

Function and Biology Details

Reaction catalysed: 
Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192934 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-hydroxy-tetrahydrodipicolinate synthase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 310 amino acids
Theoretical weight: 34.18 KDa
Source organism: Campylobacter jejuni
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9PPB4 (Residues: 1-298; Coverage: 100%)
Gene names: Cj0806, dapA
Sequence domains: Dihydrodipicolinate synthetase family

Ligands and Environments

7 bound ligands:
Ligand EDO 38 x EDO
Ligand MG 14 x MG
Ligand ACT 15 x ACT
Ligand PGE 8 x PGE
Ligand HIS 6 x HIS
Ligand PG4 1 x PG4
Ligand PEG 5 x PEG
1 modified residue:
Ligand KPI 6 x KPI

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: C2221
Unit cell:
a: 84.78Å b: 230.74Å c: 202.2Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.171 0.17 0.207
Expression system: Escherichia coli

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