› 7kfb

Electron Microscopy
3.9Å resolution

Ebola virus GP (mucin deleted, Makona strain) bound to antibody Fab EBOV-442

Released:
DOI: 10.2210/pdb7kfb/pdb
PDB entry 7kfb coloured by chain, front view.
PDB entry 7kfb coloured by chain, side view.
PDB entry 7kfb coloured by chain, top view.
Source organisms:
Primary publication:
Convergence of a common solution for broad ebolavirus neutralization by glycan cap-directed human antibodies.
Murin CD, Gilchuk P, Ilinykh PA, Huang K, Kuzmina N, Shen X, Bruhn JF, Bryan AL, Davidson E, Doranz BJ, Williamson LE, Copps J, Alkutkar T, Flyak AI, Bukreyev A, Crowe JE, Ward AB
Cell Rep 35 108984 (2021)
PMID: 33852862
Related structures: EMD-22848

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-211933 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (6 distinct):
GP1 Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 313 amino acids
Theoretical weight: 34.98 KDa
Source organism: Ebola virus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q05320 (Residues: 32-309; Coverage: 43%)
Gene name: GP
Sequence domains: Filovirus glycoprotein
Antibody Fab EBOV-442 heavy chain Chains: G, H, I
Molecule details ›
Chains: G, H, I
Length: 240 amino acids
Theoretical weight: 25.29 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
Antibody Fab EBOV-442 light chain Chains: J, K, L
Molecule details ›
Chains: J, K, L
Length: 215 amino acids
Theoretical weight: 23.72 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
GP2 Chains: D, E, F
Molecule details ›
Chains: D, E, F
Length: 203 amino acids
Theoretical weight: 22.16 KDa
Source organism: Ebola virus
Expression system: Homo sapiens
UniProt:
  • Canonical: Q05320 (Residues: 461-629; Coverage: 26%)
Gene name: GP

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: NAG
Carbohydrate polymer
1 bound ligand:
Ligand NAG 6 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.9Å
Relevant EMDB volumes: EMD-22848
Expression system: Homo sapiens

Quick links

Citations

3 review citations

Filovirus Neutralising Antibodies: Mechanisms of Action and Therapeutic Application.
Hargreaves et al. (2021)
2 more

3 mentions without citation

Structural Biology Illuminates Molecular Determinants of Broad Ebolavirus Neutralization by Human Antibodies for Pan-Ebolavirus Therapeutic Development.
Murin et al. (2021)
2 more