7k86

X-ray diffraction
2.05Å resolution

Crystal Structure of Glutamyl-tRNA synthetase (gltX) from Stenotrophomonas maltophilia

Released:
Entry authors: Seattle Structural Genomics Center for Infectious Disease, Seattle Structural Genomics Center for Infectious Disease (SSGCID)

Function and Biology Details

Reaction catalysed:
ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 475 amino acids
Theoretical weight: 53.05 KDa
Source organism: Stenotrophomonas maltophilia K279a
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B2FHI7 (Residues: 1-467; Coverage: 100%)
Gene names: Smlt1441, gltX
Sequence domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-F
Spacegroup: P21
Unit cell:
a: 85.4Å b: 69.11Å c: 103.82Å
α: 90° β: 110.06° γ: 90°
R-values:
R R work R free
0.183 0.182 0.228
Expression system: Escherichia coli BL21(DE3)