› 7k5o

X-ray diffraction
2.16Å resolution

Bst DNA polymerase I time-resolved structure, 1 min post dATP addition

Released:
DOI: 10.2210/pdb7k5o/pdb
PDB entry 7k5o coloured by chain, front view.
PDB entry 7k5o coloured by chain, side view.
PDB entry 7k5o coloured by chain, top view.
Source organisms:
Primary publication:
Following replicative DNA synthesis by time-resolved X-ray crystallography.
Chim N, Meza RA, Trinh AM, Yang K, Chaput JC
Nat Commun 12 2641 (2021)
PMID: 33976175

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-121428 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase I Chain: A
Molecule details ›
Chain: A
Length: 580 amino acids
Theoretical weight: 66.23 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli
UniProt:
  • Canonical: E1C9K5 (Residues: 1-580; Coverage: 100%)
Gene name: polA
Sequence domains: DNA polymerase family A
DNA (5'-D(*GP*CP*GP*AP*TP*CP*AP*CP*GP*T)-3') Chain: P
Molecule details ›
Chain: P
Length: 10 nucleotides
Theoretical weight: 3.05 KDa
Source organism: synthetic construct
Expression system: Not provided
DNA (5'-D(P*TP*AP*CP*GP*TP*GP*AP*TP*CP*GP*CP*A)-3') Chain: T
Molecule details ›
Chain: T
Length: 16 nucleotides
Theoretical weight: 4.92 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 5 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 86.99Å b: 93.05Å c: 104.819Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.227
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

DNA Polymerase θ: A Cancer Drug Target with Reverse Transcriptase Activity.
Chen et al. (2021)
1 more