7k4r

X-ray diffraction
1.57Å resolution

Crystal structure of Kemp Eliminase HG3 K50Q

Released:

Function and Biology Details

Reaction catalysed:
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endo-1,4-beta-xylanase Chain: A
Molecule details ›
Chain: A
Length: 308 amino acids
Theoretical weight: 33.2 KDa
Source organism: Thermoascus aurantiacus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23360 (Residues: 28-329; Coverage: 100%)
Gene name: XYNA
Sequence domains: Glycosyl hydrolase family 10

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P212121
Unit cell:
a: 48.258Å b: 61.002Å c: 81.632Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.17 0.168 0.201
Expression system: Escherichia coli BL21(DE3)