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Electron Microscopy
3.4Å resolution

Cryo-EM structure of CRISPR-Cas surveillance complex with AcrIF7

Released:
Source organism: Pseudomonas aeruginosa
Entry authors: Chang L, Li Z, Gabel C
Related structures: EMD-22583

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero undecamer (preferred)
Entry contents:
5 distinct polypeptide molecules
1 distinct RNA molecule
Macromolecules (6 distinct):
CRISPR-associated endonuclease Cas6/Csy4 Chain: C
Molecule details ›
Chain: C
Length: 187 amino acids
Theoretical weight: 21.43 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02MM2 (Residues: 1-187; Coverage: 100%)
Gene names: PA14_33300, cas6f, csy4
Sequence domains: CRISPR-associated protein (Cas_Csy4)
CRISPR-associated protein Csy2 Chain: B
Molecule details ›
Chain: B
Length: 327 amino acids
Theoretical weight: 36.24 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02MM0 (Residues: 1-327; Coverage: 100%)
Gene names: PA14_33320, csy2
Sequence domains: CRISPR-associated protein (Cas_Csy2)
CRISPR-associated protein Csy3 Chains: D, E, F, G, H, I
Molecule details ›
Chains: D, E, F, G, H, I
Length: 342 amino acids
Theoretical weight: 37.58 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02MM1 (Residues: 1-342; Coverage: 100%)
Gene names: PA14_33310, csy1-3, csy3
Sequence domains: CRISPR-associated protein (Cas_Csy3)
CRISPR-associated protein Csy1 Chain: A
Molecule details ›
Chain: A
Length: 434 amino acids
Theoretical weight: 49.19 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q02ML9 (Residues: 1-434; Coverage: 100%)
Gene names: PA14_33330, csy1
Sequence domains: CRISPR-associated protein (Cas_Csy1)
AcrF7 Chain: J
Molecule details ›
Chain: J
Length: 83 amino acids
Theoretical weight: 9.05 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B3G1L5 (Residues: 1-83; Coverage: 100%)
Gene name: PACL_0680
RNA (61-MER) Chain: M
Molecule details ›
Chain: M
Length: 61 nucleotides
Theoretical weight: 19.54 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.4Å
Relevant EMDB volumes: EMD-22583
Expression system: Escherichia coli BL21(DE3)