7jv5

Electron Microscopy
3Å resolution

Cryo-EM structure of SKF-81297-bound dopamine receptor 1 in complex with Gs protein

Released:

Function and Biology Details

Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
D(1A) dopamine receptor Chain: R
Molecule details ›
Chain: R
Length: 502 amino acids
Theoretical weight: 55.67 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P21728 (Residues: 1-446; Coverage: 100%)
Gene name: DRD1
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Guanine nucleotide-binding protein G(s) subunit alpha isoforms short Chain: A
Molecule details ›
Chain: A
Length: 393 amino acids
Theoretical weight: 45.62 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P63092 (Residues: 2-394; Coverage: 100%)
  • Best match: P63092-4 (Residues: 2-395)
Gene names: GNAS, GNAS1, GSP
Sequence domains: G-protein alpha subunit
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1 Chain: B
Molecule details ›
Chain: B
Length: 354 amino acids
Theoretical weight: 39.08 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P62873 (Residues: 2-340; Coverage: 100%)
Gene name: GNB1
Sequence domains: WD domain, G-beta repeat
Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2 Chain: G
Molecule details ›
Chain: G
Length: 67 amino acids
Theoretical weight: 7.43 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P59768 (Residues: 2-68; Coverage: 94%)
Gene name: GNG2
Nanobody 35 Chain: N
Molecule details ›
Chain: N
Length: 135 amino acids
Theoretical weight: 14.85 KDa
Source organism: synthetic construct
Expression system: Escherichia coli

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution:
Relevant EMDB volumes: EMD-22493
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli