7jth

Electron Microscopy
4Å resolution

Cryo-EM structure of unliganded octameric prenyltransferase domain of Phomopsis amygdali fusicoccadiene synthase

Released:
Source organism: Diaporthe amygdali
Entry authors: Faylo JL, van Eeuwen T, Murakami K, Christianson DW
Related structures: EMD-22473

Function and Biology Details

Reactions catalysed:
Geranylgeranyl diphosphate = fusicocca-2,10(14)-diene + diphosphate
(2E,6E)-farnesyl diphosphate + isopentenyl diphosphate = diphosphate + geranylgeranyl diphosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Fusicoccadiene synthase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 739 amino acids
Theoretical weight: 83.88 KDa
Source organism: Diaporthe amygdali
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A2PZA5 (Residues: 1-719; Coverage: 100%)
Gene names: PaFS, orf1
Sequence domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution:
Relevant EMDB volumes: EMD-22473
Expression system: Escherichia coli BL21(DE3)