7jrj

Electron Microscopy
3.03Å resolution

Chlamydomonas reinhardtii radial spoke head and neck (recombinant)

Released:

Function and Biology Details

Reaction catalysed:
ATP + nucleoside diphosphate = ADP + nucleoside triphosphate
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
Entry contents:
14 distinct polypeptide molecules
Macromolecules (14 distinct):
Radial spoke protein 10 Chain: E
Molecule details ›
Chain: E
Length: 216 amino acids
Theoretical weight: 23.55 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q27YU4 (Residues: 1-216; Coverage: 100%)
Gene names: CHLREDRAFT_185792, CHLRE_01g005450v5, RSP10
Sequence domains: MORN repeat
Flagellar radial spoke protein 1 Chain: F
Molecule details ›
Chain: F
Length: 814 amino acids
Theoretical weight: 87.87 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q27YU0 (Residues: 1-814; Coverage: 100%)
Gene names: CHLREDRAFT_196412, RSP1
Sequence domains: MORN repeat
Nucleoside diphosphate kinase 6 Chain: K
Molecule details ›
Chain: K
Length: 586 amino acids
Theoretical weight: 61.42 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q69B19 (Residues: 1-586; Coverage: 100%)
Gene names: CHLREDRAFT_139197, CHLRE_16g654300v5, RSP23
Sequence domains:
Flagellar radial spoke protein 2 Chain: I
Molecule details ›
Chain: I
Length: 738 amino acids
Theoretical weight: 77.45 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q6UBQ3 (Residues: 1-738; Coverage: 100%)
Gene names: CHLREDRAFT_186281, RSP2
Sequence domains: Dpy-30 motif
unknown protein Chain: J
Molecule details ›
Chain: J
Length: 4 amino acids
Theoretical weight: 358 Da
Source organism: Chlamydomonas reinhardtii
Expression system: Spodoptera frugiperda
Flagellar radial spoke protein 3 Chain: G
Molecule details ›
Chain: G
Length: 362 amino acids
Theoretical weight: 39.36 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: P12759 (Residues: 160-516; Coverage: 69%)
Gene name: RSP3
Flagellar radial spoke protein 5 Chain: H
Molecule details ›
Chain: H
Length: 527 amino acids
Theoretical weight: 55.93 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q27YU7 (Residues: 1-527; Coverage: 100%)
Gene names: CHLREDRAFT_190792, RSP5
Sequence domains: Aldo/keto reductase family
Radial spoke protein 9 Chains: A, B
Molecule details ›
Chains: A, B
Length: 269 amino acids
Theoretical weight: 29.57 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q27YU5 (Residues: 1-269; Coverage: 100%)
Gene names: CHLREDRAFT_182960, CHLRE_07g330200v5, RSP9
Flagellar radial spoke protein 4 Chain: C
Molecule details ›
Chain: C
Length: 466 amino acids
Theoretical weight: 49.9 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q01656 (Residues: 1-465; Coverage: 100%)
Gene name: RSP4
Sequence domains: Radial spokehead-like protein
Flagellar radial spoke protein 6 Chain: D
Molecule details ›
Chain: D
Length: 459 amino acids
Theoretical weight: 48.88 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q01657 (Residues: 1-459; Coverage: 100%)
Gene name: RSP6
Sequence domains: Radial spokehead-like protein
Flagellar radial spoke protein 6 Chain: L
Molecule details ›
Chain: L
Length: 6 amino acids
Theoretical weight: 529 Da
Source organism: Chlamydomonas reinhardtii
Expression system: Spodoptera frugiperda
Flagellar radial spoke protein 10 Chain: M
Molecule details ›
Chain: M
Length: 5 amino acids
Theoretical weight: 444 Da
Source organism: Chlamydomonas reinhardtii
Expression system: Spodoptera frugiperda
Flagellar radial spoke protein 1 Chain: N
Molecule details ›
Chain: N
Length: 89 amino acids
Theoretical weight: 7.59 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Spodoptera frugiperda
Flagellar radial spoke protein 2 Chain: O
Molecule details ›
Chain: O
Length: 69 amino acids
Theoretical weight: 5.89 KDa
Source organism: Chlamydomonas reinhardtii
Expression system: Spodoptera frugiperda

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 3.03Å
Relevant EMDB volumes: EMD-22446
Expression system: Spodoptera frugiperda