› 7jnh

X-ray diffraction
2.89Å resolution

Crystal structure of a double-ENE RNA stability element in complex with a 28-mer poly(A) RNA

Released:
DOI: 10.2210/pdb7jnh/pdb
PDB entry 7jnh coloured by chain, front view.
PDB entry 7jnh coloured by chain, side view.
PDB entry 7jnh coloured by chain, top view.
Source organisms:
Primary publication:
RNA stabilization by a poly(A) tail 3'-end binding pocket and other modes of poly(A)-RNA interaction.
Torabi SF, Vaidya AT, Tycowski KT, DeGregorio SJ, Wang J, Shu MD, Steitz TA, Steitz JA
Science 371 (2021)
PMID: 33414189

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-197255 (preferred)
Entry contents:
2 distinct RNA molecules
Macromolecules (2 distinct):
28-mer poly(A) RNA Chain: A
Molecule details ›
Chain: A
Length: 28 nucleotides
Theoretical weight: 9.17 KDa
Source organism: synthetic construct
Expression system: Not provided
Core double ENE RNA (Xtal construct) from Oryza sativa transposon,Core double ENE RNA (Xtal construct) from Oryza sativa transposon Chain: B
Molecule details ›
Chain: B
Length: 86 nucleotides
Theoretical weight: 27.54 KDa
Source organism: Oryza sativa
Expression system: Not provided

Ligands and Environments

4 bound ligands:
Ligand SR 6 x SR
Ligand NCO 12 x NCO
Ligand SPD 1 x SPD
Ligand MG 3 x MG
1 modified residue:
Ligand CCC 1 x CCC

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P6422
Unit cell:
a: 93.973Å b: 93.973Å c: 202.171Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.186 0.184 0.225
Expression system: Not provided

Quick links

Citations

3 review citations

Let It Go: HIV-1 cis-Acting Repressive Sequences.
Ostermann et al. (2021)
2 more

1 mention without citation

A riboswitch separated from its ribosome-binding site still regulates translation.
Schroeder et al. (2023)