› 7jj0

X-ray diffraction
2.25Å resolution

GTP-specific succinyl-CoA synthetase complexed with Mg-GMPPCP

Released:
DOI: 10.2210/pdb7jj0/pdb
PDB entry 7jj0 coloured by chain, front view.
PDB entry 7jj0 coloured by chain, side view.
PDB entry 7jj0 coloured by chain, top view.
Source organism: Sus scrofa
Primary publication:
Second distinct conformation of the phosphohistidine loop in succinyl-CoA synthetase
Huang J, Fraser ME
Acta Crystallogr. D Biol. Crystallogr. 77 357-368 (2021)

Function and Biology Details

Reactions catalysed: 
GTP + succinate + CoA = GDP + phosphate + succinyl-CoA
ATP + succinate + CoA = ADP + phosphate + succinyl-CoA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127591 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Succinate--CoA ligase [ADP/GDP-forming] subunit alpha, mitochondrial Chains: A, C
Molecule details ›
Chains: A, C
Length: 305 amino acids
Theoretical weight: 32.09 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O19069 (Residues: 42-346; Coverage: 88%)
Gene name: SUCLG1
Sequence domains:
Succinate--CoA ligase [GDP-forming] subunit beta, mitochondrial Chains: B, D
Molecule details ›
Chains: B, D
Length: 395 amino acids
Theoretical weight: 42.64 KDa
Source organism: Sus scrofa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53590 (Residues: 39-433; Coverage: 91%)
Gene name: SUCLG2
Sequence domains:

Ligands and Environments

2 bound ligands:
Ligand GCP 1 x GCP
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P32
Unit cell:
a: 99.311Å b: 99.311Å c: 133.512Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.236 0.235 0.265
Expression system: Escherichia coli BL21(DE3)

Quick links