› 7ji2

X-ray diffraction
1.95Å resolution

Crystal Structure of H2-Kb in complex with a OVA mutant peptide

Released:
DOI: 10.2210/pdb7ji2/pdb
PDB entry 7ji2 coloured by chain, front view.
PDB entry 7ji2 coloured by chain, side view.
PDB entry 7ji2 coloured by chain, top view.
Source organisms:
Primary publication:
Pre-T cell receptors topologically sample self-ligands during thymocyte β-selection.
Li X, Mizsei R, Tan K, Mallis RJ, Duke-Cohan JS, Akitsu A, Tetteh PW, Dubey A, Hwang W, Wagner G, Lang MJ, Arthanari H, Wang JH, Reinherz EL
Science 371 181-185 (2021)
PMID: 33335016

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-135057 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
H-2 class I histocompatibility antigen, K-B alpha chain Chains: A, D
Molecule details ›
Chains: A, D
Length: 281 amino acids
Theoretical weight: 32.39 KDa
Source organism: Mus musculus bactrianus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01901 (Residues: 22-301; Coverage: 81%)
Gene names: H2-K, H2-K1
Sequence domains:
Beta-2-microglobulin Chains: B, E
Molecule details ›
Chains: B, E
Length: 100 amino acids
Theoretical weight: 11.84 KDa
Source organism: Mus musculus bactrianus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01887 (Residues: 21-119; Coverage: 100%)
Gene name: B2m
Sequence domains: Immunoglobulin C1-set domain
OVA mutant peptide Chains: C, F
Molecule details ›
Chains: C, F
Length: 8 amino acids
Theoretical weight: 987 Da
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand GOL 8 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 66.427Å b: 90.011Å c: 89.674Å
α: 90° β: 111.7° γ: 90°
R-values:
R R work R free
0.191 0.17 0.185
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

4 review citations

The perception and response of T cells to a changing environment are based on the law of initial value.
Huseby et al. (2022)
3 more

1 mention without citation

Pre-T cell receptors topologically sample self-ligands during thymocyte β-selection.
Li et al. (2021)