› 7jfs

X-ray diffraction
4.6Å resolution

The structure of the CBM32-1, CBM32-2, and M60 catalytic domains from Clostridium perfringens ZmpB

Released:
DOI: 10.2210/pdb7jfs/pdb
PDB entry 7jfs coloured by chain, front view.
PDB entry 7jfs coloured by chain, side view.
PDB entry 7jfs coloured by chain, top view.
Source organism: Clostridium perfringens ATCC 13124
Primary publication:
Architecturally complex O-glycopeptidases are customized for mucin recognition and hydrolysis.
Pluvinage B, Ficko-Blean E, Noach I, Stuart C, Thompson N, McClure H, Buenbrazo N, Wakarchuk W, Boraston AB
Proc Natl Acad Sci U S A 118 (2021)
PMID: 33658366

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101662 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Peptidase M60 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 981 amino acids
Theoretical weight: 112.01 KDa
Source organism: Clostridium perfringens ATCC 13124
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2YN38 (Residues: 46-1003; Coverage: 58%)
Gene name: CPF_1489
Sequence domains:

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-002
Spacegroup: P61
Unit cell:
a: 185.743Å b: 185.743Å c: 81.591Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.263 0.261 0.309
Expression system: Escherichia coli BL21(DE3)

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Citations

4 review citations

Carbohydrate-active enzymes (CAZymes) in the gut microbiome.
Wardman et al. (2022)
3 more