› 7gsp

X-ray diffraction
2Å resolution

RIBONUCLEASE T1/2',3'-CGPS, NON-PRODUCTIVE

Released:
DOI: 10.2210/pdb7gsp/pdb
PDB entry 7gsp coloured by chain, front view.
PDB entry 7gsp coloured by chain, side view.
PDB entry 7gsp coloured by chain, top view.
Source organism: Aspergillus oryzae
Primary publication:
Hydrolysis of a slow cyclic thiophosphate substrate of RNase T1 analyzed by time-resolved crystallography.
Zegers I, Loris R, Dehollander G, Fattah Haikal A, Poortmans F, Steyaert J, Wyns L
Nat Struct Biol 5 280-3 (1998)
PMID: 9546218

Function and Biology Details

Reaction catalysed: 
(1a) (RNA) containing guanosine = (RNA fragment)-3'-guanosine-2',3'-cyclophosphate + a 5'-hydroxy-ribonucleotide-3'-(RNA fragment)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132808 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Guanyl-specific ribonuclease T1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 104 amino acids
Theoretical weight: 11.09 KDa
Source organism: Aspergillus oryzae
Expression system: Escherichia coli
UniProt:
  • Canonical: P00651 (Residues: 27-130; Coverage: 100%)
Gene names: AO090011000118, rntA
Structure domains: Microbial ribonucleases

Ligands and Environments

2 bound ligands:
Ligand PO4 2 x PO4
Ligand SGP 2 x SGP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ENRAF-NONIUS
Spacegroup: P41212
Unit cell:
a: 58.5Å b: 58.5Å c: 133.4Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.193 0.228
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Nucleases: diversity of structure, function and mechanism.
Yang W. (2011)
3 more

1 mention without citation

Modulating protein-protein interactions with small molecules: the importance of binding hotspots.
Thangudu et al. (2012)