7fax

X-ray diffraction
1.8Å resolution

Complex structure of TbLeo1 and LW domain from Trypanosoma brucei

Released:
Entry authors: Liao S, Gao J, Tu X

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PWWP domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 111 amino acids
Theoretical weight: 12.41 KDa
Source organism: Trypanosoma brucei brucei TREU927
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q586Y0 (Residues: 251-358; Coverage: 28%)
Gene names: 28H13.90, Tb927.2.3480
RNA polymerase-associated protein LEO1 Chain: B
Molecule details ›
Chain: B
Length: 18 amino acids
Theoretical weight: 2 KDa
Source organism: Trypanosoma brucei brucei TREU927
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'
UniProt:
  • Canonical: Q38DC5 (Residues: 71-86; Coverage: 3%)
Gene name: Tb09.211.3840

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL18U1
Spacegroup: I4
Unit cell:
a: 79.919Å b: 79.919Å c: 38.843Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.215 0.238
Expression system: Escherichia coli 'BL21-Gold(DE3)pLysS AG'