› 7f9d

X-ray diffraction
2.5Å resolution

Homo sapiens Prolyl-tRNA Synthetase (HsPRS) in Complex with L-proline and compound L96

Released:
DOI: 10.2210/pdb7f9d/pdb
PDB entry 7f9d coloured by chain, front view.
PDB entry 7f9d coloured by chain, side view.
PDB entry 7f9d coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Targeting prolyl-tRNA synthetase via a series of ATP-mimetics to accelerate drug discovery against toxoplasmosis.
Yogavel M, Bougdour A, Mishra S, Malhotra N, Chhibber-Goel J, Bellini V, Harlos K, Laleu B, Hakimi MA, Sharma A
PLoS Pathog 19 e1011124 (2023)
PMID: 36854028

Function and Biology Details

Reactions catalysed: 
ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-tRNA(Pro)
ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139773 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional glutamate/proline--tRNA ligase Chains: A, B
Molecule details ›
Chains: A, B
Length: 505 amino acids
Theoretical weight: 57.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P07814 (Residues: 1008-1512; Coverage: 33%)
Gene names: EPRS, EPRS1, GLNS, PARS, PIG32, QARS, QPRS
Sequence domains:

Ligands and Environments

5 bound ligands:
Ligand 1UI 2 x 1UI
Ligand ZN 2 x ZN
Ligand CA 4 x CA
Ligand CL 6 x CL
Ligand PRO 2 x PRO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SOLEIL BEAMLINE PROXIMA 2
Spacegroup: P212121
Unit cell:
a: 70.49Å b: 106.13Å c: 145.55Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.187 0.263
Expression system: Escherichia coli

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